RESUMO
The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil.
Assuntos
Animais , Masculino , Infecções por Actinobacillus/veterinária , Actinobacillus seminis/isolamento & purificação , Epididimite/veterinária , Doenças das Cabras/microbiologia , Orquite/veterinária , Infecções por Actinobacillus/complicações , Infecções por Actinobacillus/microbiologia , Actinobacillus seminis/classificação , Actinobacillus seminis/genética , Brasil , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Epididimite/complicações , Epididimite/microbiologia , Cabras , Orquite/complicações , Orquite/microbiologia , /genética , Análise de Sequência de DNARESUMO
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
Assuntos
Animais , Feminino , Camundongos , Infecções por Actinobacillus/microbiologia , Actinobacillus pleuropneumoniae , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Toxina da Cólera/química , Proteínas Hemolisinas/imunologia , Imunização Secundária , Camundongos Endogâmicos ICR , Plantas Geneticamente Modificadas , Zea mays/genéticaRESUMO
Actinobacillus.actinomycetemcomitans es un bacilo Gram negativo, un patógeno virulento capaz de invadir las células epitelialesgingivales.Es agente etiológico de enfermedades periodontales destructivas y responsable de la Periodontitis Juvenil Localizada, aunque nola única causa de esta enfermedad. Es importante conocer el diagnóstico microbiológico para su estudio así como el tratamientoy terapia más eficiente para su destrucción.
Actinobacillus actinomycetemcomitans, is a Gram negative rod, a virulent pathogen able to invade the gingival epithelial cells.It is the cause of destructives periodontal diseases and responsible of Localized Juvenile Periodontitis, although it is not the onlycause of this disease.It is important to know its microbiological diagnostic ,and also the most efficient treatment and therapy to destroy it.
Assuntos
Humanos , Aggregatibacter actinomycetemcomitans , Infecções por Actinobacillus/microbiologia , Periodontite Agressiva/etiologia , Periodontite Agressiva/microbiologia , Fatores de VirulênciaAssuntos
Masculino , Feminino , Humanos , Infecções por Actinobacillus/microbiologia , Aggregatibacter actinomycetemcomitans/crescimento & desenvolvimento , Aggregatibacter actinomycetemcomitans/imunologia , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Aggregatibacter actinomycetemcomitans/patogenicidadeAssuntos
Humanos , Masculino , Feminino , Aggregatibacter actinomycetemcomitans/crescimento & desenvolvimento , Aggregatibacter actinomycetemcomitans/imunologia , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Aggregatibacter actinomycetemcomitans/patogenicidade , Infecções por Actinobacillus/microbiologiaRESUMO
The aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. A total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. The following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. Samples of subgingival plaque were collected for culture and for differential counts of microbial morphotypes. In disease sites the most frequently isolated were: Prevotella intermedia/nigrescens (65), Porphyromonas gingivalis (23), Actinobacillus actinomycetemcomitans (23), Fusobacterium nucleatum (10) and Peptostreptococcus sp. (31). The aerobic gram-positive microflora was predominant in healthy sites. Significant differences were observed in microbial morphotypes between healthy and disease sites: cocci 18.71 and 78.90, motile rods 46.12 and 16.70, total spirochetes 26.48 and 2.80, respectively. The presence of motile rods, spirochetes and P. intermedia/nigrescens were the parameters with most sensitivity to suspect periodontal disease. There were significant differences in the subgingival microflora between healthy and disease sites in patients with moderate and severe periodontitis.